The abrupt emergence and spread of the COVID-19 pandemic have given rise to reflections on the relationship between humans and other lives on earth, isolation and openness, and between the individual and the collective. The Red Brick Art Museum's first exhibition of the year invites people to examine the current situation by drawing from the past events and to look at the possibilities of building a new order for the world tomorrow.
https://t.cn/A6UfjZtb
https://t.cn/A6UfjZtb
四川大学胃肠癌中心国家重点实验室用美国invigentech（英克)转染试剂成功转染DC2.4细胞(小鼠骨髓来源树突状细胞)发表文章已见刊
2020-1-13 四川大学胃肠癌中心国家重点实验室用美国invigentech（英克）公司
INVI DNA RNA Transfection Reagent转染试剂 成功转染DC2.4细胞（小鼠骨髓来源树突状细胞)发表文章已经见刊。
2020-1-13 四川大学胃肠癌中心国家重点实验室用美国invigentech（英克）公司
INVI DNA RNA Transfection Reagent转染试剂 成功转染DC2.4细胞（小鼠骨髓来源树突状细胞)发表文章已经见刊。
云南省灵长类生物医学研究重点实验室; 灵长类转化医学研究所,2020-4-1发表文章用Invigentech公司INVI DNA转染试剂共转染5组质粒pCDNA和pmirGLO，p497 25g和pGLO-VAPBm，p497和pGLO-VAPB，p497 25g和pGLO-musVapb，p497和pGLO-musVapb到Human 293T 细胞，内容如下：
Human 293T cells was cultured in DMEM high glucose medium with 10% FBS, 1% NEAA. 500 µL medium was inoculated to each well with 24 well plate. 2 µg p497 25g and 2 µg pGLO-VAPB were added to one well as a group of co-transfection. Similarly, empty pCDNA and empty pmirGLO, p497 25g and pGLO-VAPBm, p497 and pGLO-VAPB, p497 25g and pGLO-musVapb, p497 and pGLO-musVapb formed other fifive groups of co-transfection, re spectively. Then add and mix 2 µL of DNA-INVI DNA Transfection Reagent (Invigentech, Carlsbad, CA 92008, USA) in each well. In the experiments, these 6 groups of co-transfections were performed, and each group had three biological replicates. TransDetect double-luciferase Reporter Assay Kit (Beijing TransGen Biotech, Beijing, China) was used to detect luciferase activities for each of the biological replicates with three technical repeats. The mean value of the three technical repeats was used as the value of one biological replicate. The luciferase activities of difffferent groups were then compared with two tailed t-tests.
www.ivigt.com
Human 293T cells was cultured in DMEM high glucose medium with 10% FBS, 1% NEAA. 500 µL medium was inoculated to each well with 24 well plate. 2 µg p497 25g and 2 µg pGLO-VAPB were added to one well as a group of co-transfection. Similarly, empty pCDNA and empty pmirGLO, p497 25g and pGLO-VAPBm, p497 and pGLO-VAPB, p497 25g and pGLO-musVapb, p497 and pGLO-musVapb formed other fifive groups of co-transfection, re spectively. Then add and mix 2 µL of DNA-INVI DNA Transfection Reagent (Invigentech, Carlsbad, CA 92008, USA) in each well. In the experiments, these 6 groups of co-transfections were performed, and each group had three biological replicates. TransDetect double-luciferase Reporter Assay Kit (Beijing TransGen Biotech, Beijing, China) was used to detect luciferase activities for each of the biological replicates with three technical repeats. The mean value of the three technical repeats was used as the value of one biological replicate. The luciferase activities of difffferent groups were then compared with two tailed t-tests.
www.ivigt.com
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