【Newsen】[ITZY新专《GUESS WHO》刷新自身初动记录]8日，ITZY刷新了自己的初动记录。于4月30日发行的迷你专辑《GUESS WHO》初动销量(以H榜为基准)达到了20万130张，超过了去年八月《Not Shy》14万4355张的初动销量，刷新了自身最高记录。同时新曲也在国内外多个榜单上都表现不俗，延续了人气上升的气势，展现了压倒性的存在感。#ITZY[超话]#
东京场的返场造型真的好看！！！！不知道是因为妆容还是灯光的原因 整场下来秀子的眼睛都是亮晶晶水汪汪的[抱一抱][抱一抱][抱一抱] 真好看[抱一抱][抱一抱][抱一抱] 好怀念长头发的小五哦 一会觉得勇子是全团最女的 一会又觉得她是全团唯一一个男的 奇了个怪了 最后返场唱hope not 真的太感动啦 hope not真的好听疯了[抱一抱][抱一抱][抱一抱]
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Unfortunately, we could not test K300H because the protein aggregated. K300R gave just a hint of binding only at pH 9.0, but no quantitative analysis could be done. This is not surprising because of the very high Km of K300R (Table 1). Taken together, we conclude that the Li+ binding site of NhaA is formed by Asp-163, Asp-164, Asp-133, and Thr-132 and that Lys-300 dramatically affects the pH response of NhaA in addition to being involved in its antiporter activity.
Unfortunately, we could not test K300H because the protein aggregated. K300R gave just a hint of binding only at pH 9.0, but no quantitative analysis could be done. This is not surprising because of the very high Km of K300R (Table 1). Taken together, we conclude that the Li+ binding site of NhaA is formed by Asp-163, Asp-164, Asp-133, and Thr-132 and that Lys-300 dramatically affects the pH response of NhaA in addition to being involved in its antiporter activity.
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